Virtual Restriction Digest

SOLUTIONS

                            10X Buffer:
                            100mM Tris pH 7.5
                        
                            MgCl2:
                            50mM
                        
                            BSA:
                            1mg/ml
                        
                            ddH2O:
                            Sterile

RESTRICTION ENZYMES

EcoRI

HindIII

BamHI

MstII

NOVEL ENZYMES

CftI

DNA SAMPLES

pLV4400

Lambda

HBB Gene

CFTR Gene

CUSTOM SAMPLES

IMPORTED SAMPLES

1. Select DNA Sample

Input Type:

2. Select Restriction Enzyme

Standard Restriction Enzymes

EcoRI G^AATTC

HindIII A^AGCTT

BamHI G^GATCC

MstII CCTNAGG

Add Custom DNA Sample

Create Custom Restriction Enzyme

Enzyme Name:

Recognition Site:

Notation Guide:

A, T, C, G - Standard nucleotides
N - Any nucleotide (A, T, C, or G)
R - Purine (A or G)
Y - Pyrimidine (C or T)
^ - Cut position (e.g., G^GATCC cuts after G)
If no ^ is specified, enzyme cuts after the first nucleotide

Examples:
EcoRI: G^AATTC (cuts between G and A)
BamHI: G^GATCC (cuts between G and G)
MstII: CCT^NAGG (cuts after T, N = any base)

🧪 Standard Lab Mode

Free exploration mode. Choose from any DNA sample and enzyme combination. Perfect for learning the basics of restriction digestion.

🔍 Case 1: CSI Analysis

Match crime scene DNA with suspect samples using EcoRI enzyme. Analyze two genetic loci to identify the perpetrator.

👨‍👩‍👦 Case 2: Paternity Test

Determine biological relationships using HindIII enzyme. Compare DNA fragments from mother, child, and potential fathers.

🩸 Case 3: Sickle Cell Screening

Screen patients for sickle cell anemia using MstII enzyme. Identify normal (AA), carrier (AS), and affected (SS) genotypes.

🧬 Case 4: Cystic Fibrosis

Detect ΔF508 mutation using novel CftI enzyme. Screen patient and family members for CF carrier status.

✂️ Virtual Restriction Digest

Case Study Mode Active

DNA Fragments After Digestion

1. Select DNA Sample

2. Select Restriction Enzyme

Standard Restriction Enzymes

Novel Restriction Enzymes

Digestion Results

Add Custom DNA Sample

Import VLDS String

Create Custom Restriction Enzyme

Notation Guide:

DNA Fragments After Digestion

1. Select DNA Sample

2. Select Restriction Enzyme

Standard Restriction Enzymes

Novel Restriction Enzymes

Digestion Results

Add Custom DNA Sample

Import VLDS String

Create Custom Restriction Enzyme

Notation Guide:

Molecular Vision: Restriction Enzyme Digestion

Restriction Digest Results

Select Experiment Mode

🧪 Standard Lab Mode

🔍 Case 1: CSI Analysis

👨‍👩‍👦 Case 2: Paternity Test

🩸 Case 3: Sickle Cell Screening

🧬 Case 4: Cystic Fibrosis

Case Briefing