Genomic
Human Genomic DNA - Complete human genome sample
Lambda
Lambda Phage DNA - Viral DNA used as control
HBB Gene
Human Beta-globin gene region - For sickle cell analysis
CFTR Gene
CFTR gene region - For cystic fibrosis detection
Crime Scene
Crime Scene DNA - Evidence from the scene
Suspect 1
Suspect 1 DNA - Reference sample
Suspect 2
Suspect 2 DNA - Reference sample
Suspect 3
Suspect 3 DNA - Reference sample
Suspect 4
Suspect 4 DNA - Reference sample
Mother
Mother DNA - Maternal reference
Child
Child DNA - Test subject
Father 1
Alleged Father 1 DNA
Father 2
Alleged Father 2 DNA
Father 3
Alleged Father 3 DNA
Father 4
Alleged Father 4 DNA
Control AA
Normal Control (AA) - No sickle cell
Control AS
Carrier Control (AS) - Sickle cell trait
Control SS
Disease Control (SS) - Sickle cell disease
Jane
Patient Jane - Unknown genotype
Alice
Patient Alice - Unknown genotype
Bob
Patient Bob - Unknown genotype
Normal Ctrl
Normal Control - No CF mutation
ΔF508 Ctrl
ΔF508 Control - CF mutation
CF Mother
CF Mother - Carrier status unknown
CF Father
CF Father - Carrier status unknown
CF Patient
CF Patient - Diagnosis unknown
CF Sibling
CF Sibling - Status unknown
PCR Reagents
Master Mix
PCR Master Mix - Contains Taq polymerase, dNTPs, MgCl2, and buffer
Fwd Primer
Forward Primer (5' → 3') - Binds to template DNA to initiate synthesis
Rev Primer
Reverse Primer (5' → 3') - Binds to complementary strand
Micropipettes
Pipettes - P20 (2-20μL), P200 (20-200μL), P1000 (100-1000μL)
Test Tube Workspace
Tube 1: Empty
Tube 2: Empty
Tube 3: Empty
Tube 4: Empty
Tube 5: Empty
Tube 6: Empty
Centrifuge
0/6 tubes
Pipette Tips
1000 tips
Sterile Pipette Tips - Always use fresh tips to avoid contamination