Welcome to ELISA Virtual Lab

Today you'll develop a direct ELISA assay to detect viral antigens.

Tip: While the protocol shows suggested steps, you can experiment by adding reagents in any order to see what happens!

Reagents

Antigen Antigen
Primary Antibody Primary Ab
Secondary Antibody Secondary Ab-HRP
Blocking Buffer Blocking Buffer
TMB Substrate TMB (Colorimetric)
Amplex Red Amplex Red (Fluor)
Luminol Luminol (Lumi)